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浙产檵木叶中槲皮素和山奈酚的含量测定
引用本文:胡小玲,陈月桃,张锦晶,潘晓军. 浙产檵木叶中槲皮素和山奈酚的含量测定[J]. 光谱实验室, 2011, 28(5): 2634-2637
作者姓名:胡小玲  陈月桃  张锦晶  潘晓军
作者单位:温州医学院药学院 浙江省温州市高教园区中心北路3号325035
基金项目:浙江省新苗人才计划资助项目(2010R413032)
摘    要:
建立用反相高效液相色谱法同时测定浙产檵木叶中槲皮素和山奈酚含量的方法.采用Eclipse XDB-C18柱;柱温:25℃;流动相为乙腈-0.4%磷酸水溶液(35 ∶ 65,V/V);流速1.0mL/min;检测波长360nm.结果表明,槲皮素、山奈酚在1-100ng/μL的范围内均具有良好的线性关系(r槲皮素=0.99...

关 键 词:檵木叶  槲皮素  山奈酚  高效液相色谱法

Determination of Quercetin and Kaempferol in the Leaves of Loropetalum Chinensis(R.Br.)Oliv.from Zhejiang
HU Xiao-Ling,CHEN Yue-Tao,ZHANG Jin-Jing,PAN Xiao-Jun. Determination of Quercetin and Kaempferol in the Leaves of Loropetalum Chinensis(R.Br.)Oliv.from Zhejiang[J]. Chinese Journal of Spectroscopy Laboratory, 2011, 28(5): 2634-2637
Authors:HU Xiao-Ling  CHEN Yue-Tao  ZHANG Jin-Jing  PAN Xiao-Jun
Affiliation:HU Xiao-Ling CHEN Yue-Tao ZHANG Jin-Jing PAN Xiao-Jun(Department of Medicine,Wenzhou Medical College,Wenzhou,Zhejiang 325035,P.R.China)
Abstract:
A method based on reversed phase high performance liquid chromatography was developed to simultaneously determine the content of quercetin and kaempferol in the leaves of Loropetalum chinensis(R.Br.)Oliv.from Zhejiang.Eclipse XDB-C18 column was used at 25 ℃ with a mobile phase of acetonitrile-0.4% phosphoric acid(35∶65) at a flow rate of 1.0 mL /min.The UV wavelength of detection was at 360nm.Quercetin and kaempferol were better in linear correlation between 1—100ng/μL(rQuercetin=0.9998,rKaempferol=0.9999).The method is very simple,accurate and with good reappearance,which can be used for quality control in the leaves of Loropetalum chinensis(R.Br.)Oliv..
Keywords:Leaves of Loropetalum Chinensis(R.Br.)Oliv.  Quercetin  Kaempferod  High Performance Liquid Chromatography  
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