Development and validation of an SPE methodology combined with LC-MS/MS for the determination of four ionophores in aqueous environmental matrices |
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| Authors: | Søren Alex Bak Martin Hansen Kristine A. Krogh Asbjørn Brandt Bent Halling-Sørensen Erland Björklund |
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| Affiliation: | 1. Analytical Biosciences, Department of Pharmacy , Faculty of Health and Medical Sciences, University of Copenhagen , Universitetsparken 2, 2100 , Copenhagen , Denmark soerenbak@gmail.com;3. Analytical Biosciences, Department of Pharmacy , Faculty of Health and Medical Sciences, University of Copenhagen , Universitetsparken 2, 2100 , Copenhagen , Denmark;4. Department of Veterinary Medicine , Danish Health and Medicines Authority , Axel Heides Gade 1, 2300 , Copenhagen , Denmark |
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| Abstract: | A multi-residue analytical methodology has been established for the determination of the four ionophores: lasalocid, monensin, salinomycin and narasin in aqueous environmental matrices, using nigericin as internal standard. The samples were filtrated prior to solid phase extraction. All compounds were measured using liquid chromatography coupled to tandem mass spectrometry applying electro spray ionisation. The absolute recoveries ranged from 92 to 110% (relative standard deviation: 2–14%) for spiked river water. The final method allowed for detection of ionophores down to a few ng/L in natural water bodies with LOQs for the entire methodology being 40, 49, 67, and 14 ng/L for lasalocid, monensin, salinomycin, and narasin, respectively. |
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| Keywords: | ionophores pharmaceuticals anticoccidials antibiotics SPE LC-MS/MS |
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