On-column isomerization of sugars during high-performance liquid chromatography: analysis of the elution profile |
| |
| Authors: | Takashi Nishikawa Satomi Suzuki Hiroaki Kubo and Hideki Ohtani |
| |
| Institution: | Department of Clinical Pathology, School of Medicine, Kitasato University, 1-15-1, Kitasato, Sagamihara, Kanagawa, Japan |
| |
| Abstract: | Four monosaccharides (glucose, galactose, mannose and fructose) and one disaccharide (maltose) were subjected to high-performance liquid chromatography with UV or refractive index detection. Various profiles such as broad, tailed and splitted peaks were produced, depending on column temperature and eluent flow-rate because these saccharides underwent isomerization. In contrast,  -methylglucoside, a non-converting derivative, always produced a sharp peak. By analyzing these profiles kinetic constants of the isomerization were obtained and compared with the literature data. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
