| 线性扫描伏安法研究大豆甙元与牛血清白蛋白的相互作用 |
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| 引用本文： | 尚永辉,孙家卷,刘彬,黄怡,赵维.线性扫描伏安法研究大豆甙元与牛血清白蛋白的相互作用[J].化学研究,2013(2):159-161. |
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| 作者姓名： | 尚永辉 孙家卷 刘彬 黄怡 赵维 |
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| 作者单位： | 咸阳师范学院化学与化工学院 |
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| 基金项目： | 国家自然科学基金项目(20975081);陕西省科技计划项目(2012JQ2013,2011JM2012);陕西省电化学重点实验室项目(2010JS089);咸阳师范学院专项科研基金项目(11XSYK104,10XSYK101) |
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| 摘 要： | 采用线性扫描伏安法研究了大豆甙元与牛血清白蛋白(BSA)之间的相互作用;探讨了缓冲体系、pH、反应时间、反应温度、扫描速率等对相互作用的影响.结果表明,在pH为7.4的B-R缓冲溶液中,大豆甙元与BSA在相互作用20min后形成1∶1型的单一的非电活性超分子化合物,两者的结合常数β=8.29×105 L·mol-1,转移系数α=0.367 9,表观电子传递速率常数ks为1.39s-1.
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| 关 键 词： | 大豆甙元 牛血清白蛋白 相互作用 线性扫描伏安法 |
Study of interaction between daidzein and bovine serum albumin by linear sweep voltammetry |
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| SHANG Yong-hui,SUN Jia-juan,LIU Bin,HUANG Yi,ZHAO Wei.Study of interaction between daidzein and bovine serum albumin by linear sweep voltammetry[J].Chemical Research,2013(2):159-161. |
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| Authors: | SHANG Yong-hui SUN Jia-juan LIU Bin HUANG Yi ZHAO Wei |
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| Institution: | (School of Chemistry & Chemical Engineering,Xianyang Normal College,Xianyang 712000,Shaanxi,China) |
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| Abstract: | The interaction between daidzein and bovine serum albumin （BSA） was studied by linear sweep voltammetry, and the influences of buffer system, pH value, reaction time, tem- perature, and scanning rate on the interaction were investigated. Results show that an electro- chemically inactive supermolecular complex with a BSA/daidzein molar ratio of 1 ： 1 is genera- ted after daidzein and BSA interact in the Britton-Robinson buffer solution （pH = 7.4） for 20 min. In terms of the interaction between BSA and daidzein, the binding constant β is 8.29 ×105L·mol-1, the electrochemical parameters charge coefficient a is 0. 367 9, and the standard elec- tron transfer rate constant ks is 1.39 s-1. |
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| Keywords: | daidzein BSA interaction~ linear sweep voltammetry |
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