活性污泥合成PHA及其对磁场的响应研究

在好氧瞬时供料工艺下，采用逐步增加负荷法对活性污泥进行驯化，控制每个周期丰盛一匮乏时间比为1：3．当有机负荷为5420mgCOD·L^-1时，PHA（polyhydroxyalkanoate）含量可达48．6％；当有机负荷为720mgCOD·L^-1时，PHA的合成速率最高达到0．18mgCOD／mg CODX·h,取驯化好的污泥在不同磁场强度下进行PHA的合成，实验发现磁场对聚羟基丁酸（hydroxybutyrate，HB）和聚羟基戊酸（hydroxyvalerate，HV）的合成产生影响．磁场强度为7mT时，活性污泥中HB含量最高；在磁场强度为21mT时，活性污泥中HV含量最高；在磁场强度为11mT时，活性污泥中PHA含量最高．强度为7mT的磁场对HB聚合酶活性促进作用最大；强度为21mT的磁场对HV聚合酶活性促进作用最大．

Investigation on PHA synthesis by activated sludge and the synthesis response to magnetic field

Activated sludge was acclimated by gradually increasing organic load under aerobic dynamic feeding （ADF） conditions, and the ＂feast＂ /＂famine＂ ratio was 1 ： 3. The polyhydroxyalkanoate（PHA） content in biomass was 48.6% when the organic load increased to 5 420mgCOD ·L^-1. The PHA production rate reaches the maximum 0.18 mgCOD/mgCODX, h when the organic load was 720 mgCOD·L^-1. The magnetic field exposure influenced the biosynthesis of hydroxybutyrate（HB） and hydroxyvalerate（HV） by the acclimated activated sludge. HB content in biomass reached the maximum at 7 mT, while HV content in biomass reached the maximum at 21 mT, and PHA content in biomass reached the maximum at 11 mT. The HB polymerase was most activated at 7 mT, and the HV polymerase was most activated at 21mT.