Determination of Taxotere in human plasma by a semi-automated high-performance liquid chromatographic method. |
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| Authors: | J C Vergniol R Bruno G Montay A Frydman |
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| Institution: | Rh?ne-Poulenc Rorer Recherche Developpement, Institut de Biopharmacie, Antony, France. |
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| Abstract: | A rapid, selective and reproducible high-performance liquid chromatographic (HPLC) method with ultraviolet detection was developed for the determination of the anti-cancer agent Taxotere in biological fluids. The method involves a solid-phase extraction step (C2 ethyl microcolumns) using a Varian Advanced Automated Sample Processor (AASP) followed by reversed-phase HPLC. The validated quantitation range of the method is 10-2500 ng/ml in plasma with coefficients of variation < or = 11%. The method is also suitable for the determination of Taxotere in urine samples under the same conditions. The method was applied in a phase I tolerance study of Taxotere in cancer patients, allowing the pharmacokinetic profile of Taxotere to be established. |
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