Semiconductor gas sensors in bioreactor control |
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Authors: | Klaus-Dieter Vorlop Jürgen Werner Becke Jürgen Stock Joachim Klein |
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Institution: | Institut für Technische Chemie, TU Braunschweig, Hans-Sommer-Str. 10, D-3300 Braunschweig Federal Republic of Germany |
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Abstract: | A simple semiconductor gas sensor (TGS 812) is used for the on-line measurement and control of indole during the production of l-tryptophan from indole and l-serine with immobilized E. coli cells. Indole is estimated in the reactor gas space. In combination with an automatic indole supply system, a feed-batch process became possible. The indole concentration was monitored and kept within the optimal range (300–600 mg l?1). A simple gas-sensing electrode dipped in the reaction medium provides direct measurement of organic solvents and gases in the liquid. Such a system is suitable for on-line determination of ethanol (10–70 g l?1) during continuous production of ethanol with immobilized yeast cells. |
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