荧光探针法研究有机溶剂对DNA行为构象的影响

以溴化乙锭为荧光探针,考察了醇类、丙酮、乙腈等部分常见有机溶剂对DNA构象变化的影响,通过对荧光光谱,Scatchard图,DNA热变性曲线,并结合紫外吸收光谱等研究表明,有机溶剂使DNA的构象由B型向A型转变,但DNA并不发生解旋或降解。本实验为进一步研究的有机溶剂条件下DNA与非水溶性抗癌药物的相互作用机理提供了前提条件,同时也将有助于对某些生命生理现象的理解。

Effect of Organic Solvents on DNA Conformation by Fluorescent Probe Method

In this paper,the conformation of calf thymus DNA(DNA) is investigated in some common solvents by spectrofluorimetry.The result of fluorescence spectra and scatchard plot indicate that the binding constant between DNA and ethidium bromide (EB) will decrease with the increasing concentration of ethanol,and the number of binding sites will increase.The change of binding constant elucidate that the interaction between DNA and EB will become poor,and the change of binding sites shows that the conformation of DNA has transformed from B-form to A-form.In the course of this change,the distance between both neighboring base plan will be decreased.When the EB molecule just fit into the interval,the fluorescence intensity will be maximum.When DNA shrink further,the EB molecules will be excluded from DNA,then the fluorescence intensity will decrease.Although the conformation of DNA will be changed in organic solvents,it will not denaturate or dehelix,because the absorption of DNA will not increase greatly with the increasing concentration of ethanol.The melting temperature of DNA decreases continually as the weight fraction of ethanol concentration is increased.It shows that the DNA will be more distabilized in ethanol,so it helps to explain some phenomena of life and physiology.Meanwhile,the results offer an opportunity to further study the interaction DNA and insoluble drugs.