Fluorescein-based pI markers for capillary isoelectric focusing with laser-induced fluorescence detection |
| |
| Authors: | Slais Karel Horká Marie Novácková Jana Friedl Zdenek |
| |
| Institution: | Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Veverí 97, CZ-61142 Brno, Czech Republic. slais@iach.cz |
| |
| Abstract: | We prepared a series of low-molecular-mass fluorescent ampholytes with narrow pI range. These fluorescein-based ampholytes are detection compatible with argon laser-induced fluorescence (LIF) detection. The selected properties, important for their routine use as fluorescent pI markers, were examined. The pI values of new fluorescein-based pI markers were determined by capillary isoelectric focusing (CIEF) using currently available low-molecular-mass pI markers for CIEF with photometric detection. The examples of CIEF with fluorometric detection of new compounds together with fluorescein isothiocyanate (FITC) derivatized proteins are presented. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
