Angular dependent rayleigh scattering of Mössbauer radiation on proteins |
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Authors: | Nienhaus G U Hartmann H Parak F Heinzl J Huenges E |
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Institution: | 1.Institut für Physikalische Chemie der UniversitÄt Münster, Schlossplatz 4/7, 4400, Münster, F.R.G. ;2.Physik-Department der T.U. München, 8046, Garching, F.R.G. ; |
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Abstract: | RSMR experiments with57Fe radiation were performed on myoglobin. An areasensitive detector was employed for simultaneous angular dependent collection of the scattered quanta up to a maximum angle 2θ of 17?. Experimental data of polycrystalline and lyophilized myoglobin are compared with computer calculations of the scattering which are based on the atomic coordinates determined by X-ray structure analysis. Special attention has been paid to the influence of coherence effects from collectively moving parts of the protein. A simple model is introduced in order to take into account these segmental motions. Our first results indicate that the sizes of collectively moving segments are comparable with spheres of about 6 å in diameter in dry myoglobin. In myoglobin crystals, where the molecules are surrounded by large hydration shells, the movements appear to be correlated in segments with sizes comparable to helices. |
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