FLUIDITY AND OXYGEN PENETRATION OF LIPID VESICLES STUDIED BY FLUORESCENCE PROBES |
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| Authors: | Margaret W Geiger Nicholas J Turro |
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| Institution: | Chemistry Department, Columbia University, New York, NY 10027, U.S.A. |
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| Abstract: | Abstract. –An analysis of the temperature dependence of trans -stilbene fluorescence yield in dipalmitoyl lecithin vesicles is used to obtain activation energies. The results are interpreted in terms of bilayer fluidity through and above the phase transition. Oxygen quenching of the fluorescence of pyrenebutyric acid (incorporated in dipalmitoyl lecithin and egg lecithin vesicles) is reported as a function of temperature and bulk oxygen concentration. Above the bilayer phase transition, quenching rates (determined by oxygen quenching) decrease with decreasing temperature. A reduction in oxygen quenching is observed through the dipalmitoyl lecithin phase transition. |
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