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Fermentation of “Quick Fiber” produced from a modified corn-milling process into ethanol and recovery of corn fiber oil
Authors:Bruce S. Dien  Nick Nagle  Kevin B. Hicks  Vijay Singh  Robert A. Moreau  Melvin P. Tucker  Nancy N. Nichols  David B. Johnston  Michael A. Cotta  Quang Nguyen  Rodney J. Bothast
Affiliation:1. National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, 1815 North University Street, 61604, Peoria, IL
2. Biotechnology Division for Fuels and Chemicals, National Renewable Energy Laboratory, 1617 Cole Boulevard, 80401, Golden, CO
3. Eastern Regional Research Center, USDA, Agricultural Research Service, 19038, Wyndmoor, PA
4. Department of Agricultural Engineering, University of Illinois, 61801, Urbana, IL
5. National Corn to Ethanol Research Pilot Plant, SIU Edwardsville, 1 N. Research Drive, 62025, Edwardsville, IL
Abstract:Approximately 9% of the 9.7 billion bushels of corn harvested in the United States was used for fuel ethanol production in 2002, half of which was prepared for fermentation by dry grinding. The University of Illinois has developed a modified dry grind process that allows recovery of the fiber fractions prior to fermentation. We report here on conversion of this fiber (Quick Fiber [QF]) to ethanol. QF was analyzed and found to contain 32%wt glucans and 65%wt total carbohydrates. QF was pretreated with dilute acid and converted into ethanol using either ethanologenic Escherichia coli strain FBR5 or Saccharomyces cerevisiae. For the bacterial fermentation the liquid fraction was fermented, and for the yeast fermentation both liquid and solids were fermented. For the bacterial fermentation, the final ethanol concentration was 30 g/L, a yield of 0.44 g ethanol/g of sugar(s) initially present in the hydrolysate, which is 85% of the theoretical yield. The ethanol yield with yeast was 0.096 gal/bu of processed corn assuming a QF yield of 3.04 lb/bu. The residuals from the fermentations were also evaluated as a source of corn fiber oil, which has value as a nutraceutical. Corn fiber oil yields were 8.28%wt for solids recovered following prtetreatment.
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