狼毒乙素分子印迹膜荧光传感器的制备及在中药材检测中的应用

以狼毒大戟根部提取的狼毒乙素(ECB)为模板分子, 7-二乙氨基香豆素-3-羧酸与3-溴丙烯缩合生成的烯丙端基香豆素类化合物(DCAC)为荧光单体, 丙烯酰胺为功能单体, 乙二醇二甲基丙烯酸酯为交联剂, 偶氮二异丁腈为引发剂, 通过自由基聚合制备了具有强烈青色荧光, 并且可以选择性富集狼毒乙素的分子印迹膜荧光传感器(FL-MIF). 研究结果表明, FL-MIF吸附ECB的线性范围为1.00~50.00 μmol/L, 在线性范围内, 随着ECB浓度递增, 青色荧光(489 nm)呈现出明显的荧光猝灭响应, 狼毒乙素的检测限为0.29 μmol/L. FL-MIF对ECB具有良好的特异选择性识别性能, 印迹因子为3.58, 明显高于类似结构的2-甲氧基-4-羟基苯甲酸(MHBA)、 没食子酸(GA)和3,3'-二乙酰基-4,4'-二甲氧基-2,2',6,6'-四羟基二苯基甲烷(DDTPM). 重复再生性能优异的FL-MIF可应用于实际样品中药材狼毒大戟ECB含量的检测.

Preparation of ECB Molecularly Imprinted Film Fluorescent Sensor for Detection of Traditional Chinese Herbal Medicine†

Using Ebracteolata compound B(ECB) extracted from the root of Euphorbia Fischeriana steud as template molecule, 7-diethylamine coumarin-3-allyl terminal compounds(DCAC) synthesized by condensation reaction between 7-diethylamine coumarin-3-carboxylic acid and 3-bromopropene as fluorescent monomer, acrylamide as the functional monomer, ethylene glycol dimethacrylate as the crosslinking agent, azodiisobutyronitrile as the initiator, the molecularly imprinted fluorescent film sensor with strong cyan fluorescence was achieved through the free radical polymerization, which could selectively assemble ECB. The adsorption performance of the film showed that the linear range of ECB was 1.00—50.00 μmol/L. The cyan fluorescence(489 nm) quenching response of the film was shown obviously with the increasing concentration of ECB. The sensor could be used to detect the low concentration of ECB, with a detection limit to 0.29 μmol/L. The imprinting factor was 3.58, obviously higher than the analogues(2-methoxy-4-hydroxybenzoic acid, gallic acid and 3,3'-diacetyl-4,4'-dimethoxy-2,2',6,6'-tetrahydroxydiphenylmethane). The sensor with excellent reusability has been successfully applied to detect the content of ECB in Euphorbia fischeriana Steud.