Fragmentation studies of peptides: the formation of y ions. |
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| Authors: | P T Kenny K Nomoto R Orlando |
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| Affiliation: | Suntory Institute of Bioorganic Research, Osaka, Japan. |
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| Abstract: | The mechanism for the formation of y ions in the collision-induced dissociation (CID) spectra of protonated peptides produced by fast-atom bombardment was investigated by tandem mass spectrometry and deuterium labelling studies. The results show that a hydrogen atom attached to nitrogen and not to carbon migrates during cleavage of the amide bond. A mechanism based on these results is presented. |
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