Analysis of sulphonamides using supercritical fluid chromatography and supercritical fluid chromatography-mass spectrometry |
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| Authors: | J R Perkins D E Games J R Startin J Gilbert |
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| Institution: | Department of Chemistry, University College of Swansea, U.K. |
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| Abstract: | Packed-column supercritical fluid chromatography has been used for the separation of mixtures of sulphonamides on silica and amino-bonded stationary phases utilizing carbon dioxide with methanol modifier as the mobile phase. The effect of modifier concentration, column pressure and modifier identity on retention was also studied. Packed-column supercritical fluid chromatography-mass spectrometry (SFC-MS) of these mixtures utilizing both moving-belt and modified thermospray interfaces was also studied. The identification of sulphamethazine in a spiked porcine kidney extract was performed by SFC-MS using the moving-belt interface. |
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