Comparative evaluation of aromatic bonded phases for reversed-phase chromatography

Summary Retention factors for alkylbenzenes, polyaromatic hydrocarbons, steroids, amino acids, peptides, nucleotides, nucleosides were measured with phenylsilica, benzylsilica, phenethylsilica and octylsilica stationary phases using hydro-organic eluents containing methanol, acetonitrile or tetrahydrofuran. The retention behavior of the three arylsilicas was similar but distinctly different from that of octylsilica. The difference is attributed to the chemical properties of the organic ligates, i.e., of the alkyl- and aryl functions. The slight variations of retention behavior observed with arylsilicas on the other hand are believed to arise from differences in the pertiment phase ratios. The method used for interpretation of data is based on the analysis of the pertinent retention moduli, relative column retentivities and relative phase ratios. In addition, relationships between eluite structure and retention were examined. The results give insight into various phenomena underlying the retention process in reversed-phase chromatography.Part IV in the series on Stationary Phase Effects in Reversed-Phase Chromatography.