UPLC‐ESI/MS determination of 17 active constituents in two categorized formulas of traditional Chinese medicine,Sanhuang Xiexin Tang and Fuzi Xiexin Tang: application in comparing the differences in decoctions and macerations

A rapid and sensitive UPLC‐ESI/MS method was established and validated to determine 17 active constituents (aconitine, hypaconitine, mesaconitine, benzoylaconine, benzoylhypaconine, benzoylmesaconine, berberine, palmatine, jatrorrhizine, coptisine, baicalein, wogonin, baicalin, wogonoside, emodin, aloe‐emodin and rhein) in Sanhuang Xiexin Tang (SXT) and Fuzi Xiexin Tang (FXT), which are two classic compound recipes from Xiexin Tang categorized formulas in traditional Chinese medicien. The separation was performed on a UPLC BEH C₁₈ column gradient eluted using acetonitrile and 0.1% formic acid as mobile phase. ESI/MS was operated in positive (M + H]⁺) in selected ion recording mode for analysis of alkaloids and flavones, while in negative (M ? H]^?) selected ion recording mode for anthraquinones. All of the 17 constituents exhibited good linearity in a relatively wide concentration ranges with the lowest limits of detection of 0.38 ng/mL. All of the relative standard deviation values of intra‐ and inter‐precisions and stabilities of 17 constituents were within 5%. The method was successfully applied to determine 17 active constituents in decoctions and macerations of SXT and FXT. The results indicated that different preparative methods resulted in significant diversity in concentrations of the 17 analytes. Herb–herb interaction appeared between aconitum alkaloids in Aconiti Lateralis Radix Preparata and another three herbs. Copyright © 2013 John Wiley & Sons, Ltd.