A simple and sensitive HPLC‐UV determination of 7‐O‐succinyl macrolactin A in rat plasma and urine and its application to a pharmacokinetic study |
| |
| Authors: | Ju Myung Kim Ji Won Jung Dong Hee Kim Jae Seon Kang Chun Gyu Kim Hee Eun Kang |
| |
| Institution: | 1. College of Pharmacy and Integrated Research Institute of Pharmaceutical Sciences, The Catholic University of Korea, , Bucheon, 420‐743 South Korea;2. Research and Development Center, Daewoo Pharm. Co. Ltd., , Busan, 604‐836 South Korea;3. Department of Pharmacy, Kyungsung University, , Busan, 608‐736 South Korea;4. Department of Pharmaceutical Engineering, Inje University, , Gimhae, 621‐749 South Korea |
| |
| Abstract: | A simple, sensitive and reproducible isocratic reversed‐phase (C18) high‐performance liquid chromatography (HPLC) method was developed to determine 7‐O‐succinyl macrolactin A (SMA) in rat plasma and urine samples using UV detector set at 230 nm. Lamotrigine was used as internal standards (IS) to ensure the precision and accuracy of the method. The retention times of SMA and IS for the plasma sample were 9.2 and 4.4 min, respectively, and those for the urine samples were 7.9 and 4.3 min, respectively. The intra‐ and inter‐day variations of the analytical responses, expressed in terms of relative standard deviation, were less than 14.9%. The accuracy, in terms of average analytical recovery, ranged from 90.4 to 119%. The lower limits of quantification of SMA in rat plasma and urine samples were 0.02 and 0.1 µg/mL, respectively. This method is applicable for the pharmacokinetic studies of SMA in rats. Copyright © 2012 John Wiley & Sons, Ltd. |
| |
| Keywords: | HPLC‐UV 7‐O‐succinyl macrolactin A rat plasma rat urine pharmacokinetics |
|
|
