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Engineering Enzyme Stability and Resistance to an Organic Cosolvent by Modification of Residues in the Access Tunnel
Authors:Dr. Tana Koudelakova  Dr. Radka Chaloupkova  Dr. Jan Brezovsky  Dr. Zbynek Prokop  Dr. Eva Sebestova  Dr. Martin Hesseler  Dr. Morteza Khabiri  Maryia Plevaka  Daryna Kulik  Dr. Ivana Kuta Smatanova  Dr. Pavlina Rezacova  Dr. Rudiger Ettrich  Prof. Uwe T. Bornscheuer  Prof. Jiri Damborsky
Affiliation:1. Loschmidt Laboratories, Department of Experimental Biology and Research Centre for Toxic Compounds in the Environment, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno (Czech Republic) http://loschmidt.chemi.muni.cz/peg;2. Department of Biotechnology and Enzyme Catalysis, Institute of Biochemistry, Faculty of Mathematics and Natural Sciences, Ernst‐Moritz‐Arndt‐University, Greifswald (Germany);3. Institute of Nanobiology and Structural Biology, Academy of Sciences of the Czech Republic, Nove Hrady (Czech Republic);4. Institute of Physical Biology, University of South Bohemia, Ceske Budejovice, Nove Hrady (Czech Republic);5. Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Praha (Czech Republic)
Abstract:
Keywords:directed evolution  enzyme catalysis  enzymes  protein engineering  protein stability
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