Development of an LC/MS/MS method in order to determine arctigenin in rat plasma: its application to a pharmacokinetic study |
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| Authors: | Quanfei Zou Yuan Gu Rong Lu Tiejun Zhang Guang‐Rong Zhao Changxiao Liu Duanyun Si |
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| Institution: | 1. Key Laboratory of Systems Bioengineering, School of Chemical Engineering and Technology, Tianjin University, , Tianjin, 300072 China;2. State Key Laboratory of Drug Delivery Technology and Pharmacokinetics, Tianjin Institute of Pharmaceutical Research, , Tianjin, 300193 China |
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| Abstract: | In this study, a simple and sensitive LC/MS/MS method was developed and validated for the determination of arctigenin in rat plasma. The MS detection was performed using multiple reaction monitoring at the transitions of m/z 373.2 → 137.3 for arctigenin and m/z 187.1 → 131.0 for psoralen (internal standard) with a Turbo IonSpray electrospray in positive mode. The calibration curves fitted a good linear relationship over the concentration range of 0.2–500 ng/mL. It was found that arctigenin is not stable enough at both room temperature and ?80 °C unless mixed with methanol before storage. The validated LC/MS/MS method was successfully applied for the pharmacokinetic study of arctigenin in rats. After intravenous injection of 0.3 mg/kg arctigenin injection to rats, the maximum concentration, half‐life and area under the concentration–time curve were 323 ± 65.2 ng/mL, 0.830 ± 0.166 and 81.0 ± 22.1 h ng/mL, respectively. Copyright © 2013 John Wiley & Sons, Ltd. |
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| Keywords: | arctigenin LC/MS/MS pharmacokinetics rat plasma |
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