Surface chemistry effects on the performance of an electrochemical DNA sensor |
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Authors: | Francesco Ricci Nadia Zari Felice Caprio Simona Recine Aziz Amine Danila Moscone Giuseppe Palleschi Kevin W Plaxco |
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Institution: | aDipartimento di Scienze e Tecnologie Chimiche, Università di Roma Tor Vergata, Via della Ricerca Scientifica, 00133, Rome, Italy;bFaculté de Sciences et Techniques de Mohammadia, B.P. 146, Mohammadia, Morocco;cDepartment of Chemistry and Biochemistry and Interdepartmental Program in Biomolecular Science and Engineering, University of California Santa Barbara, Santa Barbara, CA 93106, USA |
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Abstract: | E-DNA sensors are a reagentless, electrochemical oligonucleotide sensing platform based on a redox-tag modified, electrode-bound probe DNA. Because E-DNA signaling is linked to hybridization-linked changes in the dynamics of this probe, sensor performance is likely dependent on the nature of the self-assembled monolayer coating the electrode. We have investigated this question by characterizing the gain, specificity, response time and shelf-life of E-DNA sensors fabricated using a range of co-adsorbates, including both charged and neutral alkane thiols. We find that, among the thiols tested, the positively charged cysteamine gives rise to the largest and most rapid response to target and leads to significantly improved storage stability. The best mismatch specificity, however, is achieved with mercaptoethanesulfonic and mercaptoundecanol, presumably due to the destabilizing effects of, respectively, the negative charge and steric bulk of these co-adsorbates. These results demonstrate that a careful choice of co-adsorbate chemistry can lead to significant improvements in the performance of this broad class of electrochemical DNA sensors. |
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Keywords: | DNA Sensor Electrochemical Thiol Self-assembled monolayers |
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