|
|||||
|
|
Investigation of Tomato Plant Defence Response to Tobacco Mosaic Virus by Determination of Methyl Salicylate with SPME-Capillary GC-MS |
|
| Authors: | Chunhui Deng Xiangmin Zhang Weimin Zhu Ji Qian |
| Institution: | (1) Department of Chemistry, Fudan University, Shanghai, 200433, China;(2) Academy of Agriculture Science, Shanghai, 201106, China;(3) College of Life Science, Fudan University, Shanghai, 200433, China |
| Abstract: | Headspace SPME was applied to investigation of tomato plant defence response to tobacco mosaic virus (TMV) by determination of compounds emitted from tomato plants at the extraction conditions of 25 °C and 15 min. It was found that TMV-inoculated tomato plant released large amount of methyl salicylate (MeSA) as response to TMV and MeSA concentration changed dramatically with time after inoculation. Gaseous MeSA as a signaling compound could induce in surrounding tomato plants to produce salicylic acid (SA) and synthesize and release MeSA. These results show that MeSA might be an airborne plant-signalling molecule in tomato plant response to TMV. The present method provided low detection limit of 2.0 ng L–1 and needed little sample preparation time (15 min), so the method makes it easy to find the critical times of tomato plant response to TMV by fast determination of MeSA released from tomato plant. |
| Keywords: | Gas chromatography– mass spectrometry Headspace SPME Airborne signalling molecule Methyl salicylate from tomatoes Tobacco mosaic virus |
| 本文献已被 SpringerLink 等数据库收录！ | |