| Fe3O4/TiO2 纳米材料靶上脱盐的基质辅助激光解析/电离质谱新方法研究 |
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| 引用本文： | 卫军营,张养军,谭峰,刘慧玲,王京兰,蔡耘,钱小红.Fe3O4/TiO2 纳米材料靶上脱盐的基质辅助激光解析/电离质谱新方法研究[J].分析化学,2007,35(1):1-7. |
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| 作者姓名： | 卫军营 张养军 谭峰 刘慧玲 王京兰 蔡耘 钱小红 |
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| 作者单位： | 军事医学科学院放射与辐射医学研究所北京蛋白质组研究中心,北京,102206 |
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| 基金项目： | 国家重点基础研究规划项目(No2001CB510201,2004CB520802)，国家自然科学基金(No30321003,20405017,20505018,20505019)，北京市科委重大项目(NoH030230280190)的资助 |
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| 摘 要： | 建立了一种简便、高效的靶上脱盐新方法。利用Fe3O4/TiO2磁性纳米材料对肽段的吸附作用,将其作为载体用于靶上肽段富集和脱盐。在对纳米材料使用量、浸洗条件进行优化的基础上,成功地鉴定了溶于10mol/L尿素溶液的100fmol的肌红蛋白样品,也对溶于3mol/L尿素溶液中的10fmol的肌红蛋白样品进行了成功地分析鉴定。通过对肌红蛋白样品进行预处理和质谱分析重复实验,表明该方法重现性好,且简便、高效,所需时间短,一次可同时处理多个样品,易于实现通量化,为有效地解决目前蛋白质组分析中所面临的基质辅助激光解析/离子化飞行时间质谱耐盐性差的问题提供了一种新的手段。
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| 关 键 词： | 基质辅助激光解析电离飞行时间质谱 四氧化三铁/二氧化钛纳米材料 靶上脱盐方法 |
| 修稿时间： | 2006-07-05 |
A Novel Desalting Method on Target for MALDI-TOF-MS Analysis Based on Fe3O4/TiO2 Nanoparticles |
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| Wei Jun-Ying,Zhang Yang-Jun,Tan Feng,Liu Hui-Ling,Wang Jing-Lan,Cai Yun,Qian Xiao-Hong.A Novel Desalting Method on Target for MALDI-TOF-MS Analysis Based on Fe3O4/TiO2 Nanoparticles[J].Chinese Journal of Analytical Chemistry,2007,35(1):1-7. |
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| Authors: | Wei Jun-Ying Zhang Yang-Jun Tan Feng Liu Hui-Ling Wang Jing-Lan Cai Yun Qian Xiao-Hong |
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| Institution: | Beijing Institute of Radiation Medicine Beijing Proteome Research Center, 33 Life Science Park Road,Changping District, Beijing 102206 |
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| Abstract: | A novel method for desalting directly on MALDI-TOF-MS target has been established based on the Absorption of peptides with Fe3O4/TiO2 nanoparticles. After optimization of experimental conditions, such as the amounts of nanoparticles and washing solutions, 100 fmol myoglobin digested by trypsin in 10 mol/L urea solution could be identified successfully, and the reliable result could also be obtained for 10 fmol myoglobin in 3 mol/L urea solution. The experimental results show that the method developed can be easily and effectively used for the protein identification in high salt buffer and has great potential for the large scale proteomics studies in future. |
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| Keywords: | Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry ferriferrous oxide/titanium dioxide nanoparticles desalting directly on target |
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