猪肝DFP酸催化水解反应产物的等速电泳分析

DFP酸可催化水解二异丙基氟磷酸酯（DFP）分子中的P－F键，使其断裂产生磷酸二异丙基酯和HF。根据电位滴定法和离子选择电极法对猪肝中发现的两种DFP水解酶活力测定的结果，利用毛细管等速电泳分析仪对这两种酶催化DFP水解的产物进行了定性分析。结果表明，猪肝中小分子质量的酸催化DFP水解反应时，并非水解P－F键．而是水解P－OR键，说明该酶是一种催化DFP分子中磷酯健水解的磷酯酶。

Isotachophoresis Analysis of Hydrolytic Products of DFP Catalyzed by DFPase in Porcine Liver

DFPase can hydrolyze the P-F bond of diisopropyl phosphate fluoride (DFP ) to produce diisopropyl phosphate and HF. In this article, the activity of two kinds of DFPase from porcine liver was assayed by the methods of titration and F-ion sensitive electrode. The results showed that the activity of large molecular DFPase can be determined by both methods, but the activity of small molecular DFPase can only be determined by the titration and cannot be determined by F-ion sensitive electrode. Theisotachophoresis analysis of the products of DFP hydrolyzed by the large and small molecular DFPase was studied. The results showed that the small molecular enzyme could catalyze the hydrolysis of DFP, but without the production of F-ion, and that this enzyme is one kind of phosphoesterase that hydrolyzed P-OR bond of DFP.