A high‐performance liquid chromatographic analysis and preliminary pharmacokinetic characterization of 3′‐hydroxypterostilbene in rats |
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Authors: | Jody K Takemoto Neal M Davies |
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Institution: | College of Pharmacy Department of Pharmaceutical Sciences and Pharmacology and Toxicology Graduate Program Washington State University, Pullman, WA 99164‐6534, USA |
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Abstract: | A high‐performance liquid chromatographic method was developed for the analysis of 3'‐hydroxypterostilbene. This method involves the use of a Luna® C18 column with ultraviolet detection at 325 nm. The mobile phase consisted of acetonitrile, water and formic acid (50:50:0.01, v/v/v) with a flow rate of 0.8 mL/min. The calibration curves were linear over the range 0.5–100.0 µg/mL. The mean extraction efficiency was between 97.40 and 111.16%. The precision of the assay was 0.196–14.39% (RSD%), and within 15% at the limit of quantitation (0.5 µg/mL). The bias of the assay was <16% and within 15% at the limit of quantitation. This assay was successfully applied to pre‐clinical pharmacokinetic samples from rat urine and serum. Copyright © 2009 John Wiley & Sons, Ltd. |
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Keywords: | 3′ ‐hydroxypterostilbene trans‐3 5‐dimethoxy‐3 4‐hydroxystilbene 3 5‐hydroxypterostilbene (trans)‐4‐[2‐(3 5‐dimethoxyphenyl) ethenyl]‐1 2‐benzenediol HPLC |
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