Rapid analysis of N‐linked oligosaccharides in glycoproteins (ovalbumin,ribonuclease B and fetuin) by reversed‐phase ultra‐performance liquid chromatography with fluorescence detection and electrospray ionization time‐of‐flight mass spectrometry |
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Authors: | Takamasa Kurihara Jun Zhe Min Asuka Hirata Toshimasa Toyo'oka Shinsuke Inagaki |
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Institution: | Laboratory of Analytical and Bio‐Analytical Chemistry, School of Pharmaceutical Sciences, and Global COE Program, University of Shizuoka, 52‐1 Yada, Suruga‐ku, Shizuoka 422‐8526, Japan |
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Abstract: | Rapid, selective and sensitive determination of N‐linked oligosaccharides in glycoproteins (ovalbumin, ribonuclease B and fetuin) was performed by ultra‐performance liquid chromatography (UPLC) with fluorescence (FL) and electrospray ionization time‐of‐flight mass spectrometry (ESI‐TOF‐MS). The asparaginyl‐oligosaccharide moiety was first liberated from each glycoprotein by pronase E (a proteolitic enzyme). The oligosaccharide fractions separated by gel‐permeation chromatography were labeled with 1‐pyrenesulfonyl chloride (PSC, a fluorescence reagent), separated by UPLC in a short run time, and then detected by FL and TOF‐MS. The PSC‐labeled oligosaccharides were selectively identified from the FL detection and then sensitively determined by ESI‐TOF‐MS. As the results, 15, eight and four kinds of N‐linked oligosaccharides were detected from ovalbumin, ribonuclease B and fetuin, respectively. Because the present method is rapid (within 9 min), selective and sensitive (approximate 60 fmol, S/N = 5), the determination of N‐linked oligosaccharides in various glycoproteins seems to be possible. Copyright © 2008 John Wiley & Sons, Ltd. |
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Keywords: | N‐linked oligosaccharide 1‐pyrenesulfonyl chloride ultra‐performance liquid chromatography fluorescence detection time‐of‐flight mass spectrometry ovalbumin ribonuclease B fetuin |
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