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A Facile System for Encoding Unnatural Amino Acids in Mammalian Cells |
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| Authors: | Peng R Chen Dr Dan Groff Jiantao Guo Dr Weijia Ou Dr Susan Cellitti Dr Bernhard H Geierstanger Dr Peter G Schultz Dr |
| Institution: | 1. Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 Torry Pines Road, La Jolla, CA 92037 (USA), Fax: (+1)?858‐784‐9440;2. Genomics Institute of the Novartis Research Foundation, 10675 John Jay Hopkins Drive, San Diego, CA 92121 (USA), Fax: (+1)?858‐812‐1746 |
| Abstract: | A shuttle system has been developed to genetically encode unnatural amino acids in mammalian cells using aminoacyl‐tRNA synthetases (aaRSs) evolved in E. coli. A pyrrolysyl‐tRNA synthetase (PylRS) mutant was evolved in E. coli that selectively aminoacylates a cognate nonsense suppressor tRNA with a photocaged lysine derivative. Transfer of this orthogonal tRNA–aaRS pair into mammalian cells made possible the selective incorporation of this unnatural amino acid into proteins.  |
| Keywords: | gene expression nonnatural amino acids photocaged lysine tRNA |