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Multiresidue analysis of pesticides with hydrolyzable functionality in cooked vegetables by liquid chromatography tandem mass spectrometry |
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| Authors: | Sung Joong Lee Semin Park Jin Young Choi Jae‐Han Shim Eun‐Ho Shin Jeong‐Heui Choi Soo Taek Kim A. M. Abd El‐Aty Jong Sung Jin Dong Won Bae Sung Chul Shin |
| Affiliation: | 1. Department of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju, 660‐701, Republic of Korea;2. Natural Products Chemistry Laboratory, Division of Applied Bioscience and Biotechnology, College of Agriculture and Life Science, Chonnam National University, 300 Yong‐Bong Dong, Buk‐Ku, Gwangju 500‐757, Republic of Korea;3. Department of Information and Statistics & Research Institute of National Science, Gyeongsang National University, Jinju, 660‐701, Republic of Korea;4. Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, 1 Hwayang‐dong, Kwangjin‐gu, Seoul 143‐701, Republic of Korea;5. Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, 12211‐Giza, Egypt;6. Korea Basic Science Institute Busan Center, High Technique Components & Materials Team, Gangseo‐gu, Busan, 618‐230, Republic of Korea;7. Central Laboratory, Gyeongsang National University, Jinju, 660‐701, Republic of Korea |
| Abstract: | It would be preferable for pesticide residues substituted by hydrolyzable functionality to be analyzed after cooking because their structures are apt to degrade during boiling and/or heating. A liquid chromatography–tandem mass spectrometry (LC‐MS/MS) method for the quantitative determination of 44 pesticide residues with hydrolyzable functional group in five typical vegetable widely consumed in Republic of Korea is described. The sample clean‐up was carried out according to the method of Food Code No. 83 established by the Korea Food and Drug Administration (KFDA). Zorbox XDB‐C18 column was selected for the analysis because of the best peak separation. The LC mobile phase consisted of water and 5 mm methanolic ammonium formate, which resulted in a peak shape with good symmetry at each run. Tandem mass spectroscopic (MS/MS) experiments were performed in ESI positive mode and the multiple reaction monitoring modes. A conventional matrix effect was modified to more comprehensive form 100γij (%). A high matrix effect ( |
| Keywords: | cooked vegetables residues simultaneous analysis multiclass pesticides tandem mass liquid chromatography |