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| 食品中三唑酮的酶联免疫吸附分析 | |
| 引用本文： | 王勇 李治祥. 食品中三唑酮的酶联免疫吸附分析[J]. 分析化学, 1993, 21(9): 1055-1057 |
| 作者姓名： | 王勇 李治祥 |
| 作者单位： | 农业部环境保护所，农业部环境保护所，农业部环境保护所，农业部环境保护所 天津 300191，天津 300191，天津市农科院天津 300191，天津 300191 |
| 摘 要： | 制备人血清白蛋白和三唑酮的结合物作为免疫原,免疫兔子获得了抗三唑酮的抗体,并建立了三唑酮的酶联免疫吸附分析法。此方法被成功的应用于黄瓜、梨等食品中三唑酮的分析,最低检出限为40ng/g样品,回收率为92.44％～98.18％。并与气相色谱分析结果有很好的一致性。 |
| 关 键 词： | 三唑酮 酶联免疫吸附 分析 食品 |
Enzyme-Linked Immunosorbent Assay for Triazolone in Foods |
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| Wang Yong,Li Zhixiang,Zhu Haiying,Zhang Junting. Enzyme-Linked Immunosorbent Assay for Triazolone in Foods[J]. Chinese Journal of Analytical Chemistry, 1993, 21(9): 1055-1057 | |
| Authors: | Wang Yong Li Zhixiang Zhu Haiying Zhang Junting |
| Abstract: | A human serum albumin-triazolone conjugate was synthesized as an immunogen ,the antiserum of tirazolone was produced by immunization of rabbits and used to develop an enzyme-linked immunosorbent assay (ELISA). The assay was successfully validated for application in the determination of triazolone in cucumber and pear. The detection limit was 40 ng/g sample. The recoveries were in the range of 92. 44% -98. 18% ,the results of ELISA were in accord with those of GC determination. |
| Keywords: | Triazolone Enzyme-linked immunosorbent assay. |
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