Enzymatic profiling system in a small-molecule microarray |
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Authors: | Zhu Qing Uttamchandani Mahesh Li Dongbo Lesaicherre Marie L Yao Shao Q |
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Institution: | Department of Chemistry, National University of Singapore, 3, Science Drive 3, Singapore 117543, Republic of Singapore. |
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Abstract: | reaction: see text] We have developed a microarray-based strategy for detection of three major classes of hydrolytic enzymes on the basis of their catalytic activities. This enables the sensitive detection of proteins not merely by their bindings but rather by their enzymatic activities. This may provide a valuable tool for screening, identification, and characterization of new enzymes in a high-throughput fashion. |
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