| 基于新型聚钙羧酸修饰电极的甲胎蛋白电化学免疫传感器研究 |
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| 引用本文： | 林晓,翁少煌,周剑章,刘爱林,林新华,游勇基.基于新型聚钙羧酸修饰电极的甲胎蛋白电化学免疫传感器研究[J].电化学,2012,18(4):371-376. |
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| 作者姓名： | 林晓 翁少煌 周剑章 刘爱林 林新华 游勇基 |
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| 作者单位： | 1. 福建医科大学药学院药物分析学系，福建 福州 350004； 2. 厦门大学 固体表面物理化学国家重点实验室，化学化工学院化学系，福建 厦门 361005 |
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| 基金项目： | 福建省高校产学研科技重点项目(No.2010Y4003);福建省自然科学基金项目(No.2011J05023);福建医科大学博士启动基金(No.2010BS006)资助 |
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| 摘 要： | 通过电聚合制得新型聚钙羧酸修饰电极并用于构建检测甲胎蛋白（AFP）的高灵敏电化学免疫传感器. 采用扫描电镜（SEM）、电化学交流阻抗（EIS）观察、表征修饰电极和AFP单克隆抗体（Ab1）固定前后的差异. 固定Ab1的电极与一定浓度的AFP、辣根过氧化物酶联AFP单克隆抗体（HRP-Ab2）反应，形成夹心型免疫复合物. 辣根过氧化物酶（HRP）催化3,3',5,5'-四甲基联苯胺（TMB）底物产生电流信号，实现AFP浓度的测定. 本检测方法灵敏度高，重现性好.
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| 关 键 词： | 聚钙羧酸修饰电极 甲胎蛋白 免疫传感器 |
| 收稿时间： | 2012-01-10 |
Electrochemical Immunosensor Based on Polycalconcarboxylic Acid Modified Electrode for the Determination of Alpha-Fetoprotein |
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| LIN Xiao,WENG Shao-huang,ZHOU Jian-zhang,LIU Ai-lin,LIN Xin-hua,YOU Yong-ji.Electrochemical Immunosensor Based on Polycalconcarboxylic Acid Modified Electrode for the Determination of Alpha-Fetoprotein[J].Electrochemistry,2012,18(4):371-376. |
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| Authors: | LIN Xiao WENG Shao-huang ZHOU Jian-zhang LIU Ai-lin LIN Xin-hua YOU Yong-ji |
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| Institution: | 1(1.Department of Pharmaceutical Analysis,College of Pharmacy,Fujian Medical University, Fuzhou 350004,China;2.State Key Laboratory of Physical Chemistry of Solid Surface, Department of Chemistry,College of Chemistry and Chemical Engeering, Xiamen University,Xiamen 361005,Fujian,China) |
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| Abstract: | A highly sensitive electrochemical immunosensor based on novel electropolymerized polycalconcarboxylic acid modified electrode(poly-CCA/GC) has been fabricated for the detection of α-fetoprotein(AFP).The differences of the poly-CCA/GC electrodes before and after the immobilization of AFP antibody were characterized via scanning electron microscopy(SEM) and electrochemical impedance spectroscopy(EIS).The immobilization of antibody/antigen/antibody-HRP sandwich was formed on the poly-CCA modified electrode for the determination of special AFP concentrations.The electrochemical signals generated from the HRP which catalyzed the TMB substrate were measured.Experiment results indicated that the developed immunosensor showed a good sensitivity and precision. |
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| Keywords: | polycalconcarboxylic acid modified electrode α-fetoprotein immunosensor |
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