量子点-卟啉纳米复合体系在肿瘤细胞成像中的应用

本文合成了高荧光量子产率、单分散性好的水溶性CdTe量子点(quantum dots,QDs),并与α,β,γ,δ-四(1-甲基吡啶嗡-4-基)卟吩对甲苯磺酸盐(TMPyP)组装成QDs-TMPyP纳米复合物,研究了该复合物检测DNA的机理以及肿瘤细胞成像。结果显示,QDs-TMPyP纳米复合物通过光致电子转移机制检测DNA,当CdTe QDs和CdTe QDs-TMPyP浓度低于1.0μmol/L时,HeLa肿瘤细胞存活率达92%以上,表现出低的细胞毒性。0.2μmol/L CdTe QDs-TMPyP作用于肿瘤细胞时,细胞生长状态良好,对细胞内能谱分析发现细胞内含有Cd和Te原子。CdTe QDs-TMPyP复合物比CdTe QDs更易被HeLa细胞摄取,利用量子点荧光成功实现了细胞核内成像,为宫颈癌细胞药物输送和细胞成像的深入研究打下基础。

Application of CdTe QDs-TMPyP Nanocomplex in Imaging of Tumor Cells

The solution CdTe quantum dots (Dots) with high quantum yield and good homogeneous distribution conjugate meso-tetrakis (N-methylpyridinium-4-yl) porphyrin (TMPyP) to construct QDs-TMPyP nanocomplex.The QDs-TMPyP nanocomplex was achieved to detection of DNA by photo induced electrontransfer (PIET) process.CdTe QDs and CdTe QDs-TMPyP showed a remarkable inhibition for the proliferation of cancer cells in vitro at higher concentrations,but only small effects at lower concentrations.The cells viabilities of HeLa cells were above 92％ under treated with lower than 1.0 μmol/L CdTe QDs and CdTe QDs-TMPyP for 24 h.The HeLa cancer cells treated with 0.2 μmol/L CdTe QDs-TMPyP were found to contain Cd and Te atoms by EDS analysis.The uptake of CdTe QDs-TMPyP was easier than CdTe QDs in HeLa cancer cells.The CdTe QDs-TMPyP nanocomplex was successfully achieved in HeLa cells fluorescent imaging,which lays the foundation for the drug delivery and cell imaging.