Implementation and efficiency of an automatic peak-purity-control procedure in HPLC-UV-VIS-coupling based on principal component analysis |
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Authors: | S Ebel W Mueck |
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Institution: | (1) Department of Pharmacy, Würzburg University, Am Hubland, 8700 Würzburg, FRG |
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Abstract: | Summary Based on a thorough knowledge of the actual system precision significance testing of the primary eigen values, resulting from principal component analysis of the two-dimensional data array of HPLC with photodiode-array detection, is a powerful means to uncover unresolved chromatographic peaks. The implementation of this chemometric technique for assuring peak homogeneity and results showing the efficiency for two-component peaks in regard to spectral characteristics, chromatographic resolution and absorbance ratio of the investigated compounds are presented. |
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Keywords: | HPLC/UV hyphenated techniques Principal component analysis eigenvalue Unresolved peaks peak purity (or peak homogeneity) Absorbance ratio |
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