In vivo imaging of cell morphology and cellular processes in Caenorhabditis elegans, using non-linear phenomena |
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| Authors: | G. Filippidis E.J. Gualda M. Mari K. Troulinaki C. Fotakis N. Tavernarakis |
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| Affiliation: | aInstitute of Electronic Structure and Laser, Foundation of Research and Technology-Hellas, P.O. Box 1385, 71110 Heraklion, Crete, Greece;bInstitute of Molecular Biology and Biotechnology, Foundation of Research and Technology, 71110 Heraklion, Crete, Greece;cDepartment of Physics, University of Crete, Greece |
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| Abstract: | We present the detailed imaging of structures and processes of the nematode Caenorhabditis elegans (C. elegans) using non-linear microscopy. Complementary information about the anatomy of the nematode was collected by implementing a combination of two photon excitation fluorescence (TPEF), second and third harmonic generation (SHG and THG) image contrast modes on the same microscope. Three-dimensional (3D) reconstructions of TPEF, SHG and THG images were also performed. Moreover, THG imaging technique has been tested as a potential, novel, non-destructive diagnostic tool for monitoring cellular processes in vivo, such as neuronal degeneration. |
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| Keywords: | THG SHG TPEF Imaging Caenorhabditis elegans Neurodegeneration |
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