Gene analysis of multiple oral bacteria by the polymerase chain reaction coupled with capillary polymer electrophoresis |
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| Authors: | Chenchen Liu Yoshinori Yamaguchi Shinichi Sekine Yi Ni Zhenqing Li Xifang Zhu Xiaoming Dou |
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| Institution: | 1. Engineering Research Center of Optical Instrument and System, University of Shanghai for Science and Technology, Shanghai, China;2. Institute of Photonics and Bio‐medicine (IPBM), Graduate School of Science, East China University of Science and Technology (ECUST), Shanghai, China;3. Department of Applied Physics, Graduate School of Engineering, Osaka University, Osaka, Japan;4. Division of Special Care Dentistry, Osaka University Dental Hospital, Osaka University, Osaka, Japan;5. Department of Preventive Dentistry, Osaka University Graduate School of Dentistry Osaka University, Osaka, Japan;6. School of Optoelectronic Engineering, ChangZhou Institute of Technology, Changzhou, Jiangsu, China |
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| Abstract: | Capillary polymer electrophoresis is identified as a promising technology for the analysis of DNA from bacteria, virus and cell samples. In this paper, we propose an innovative capillary polymer electrophoresis protocol for the quantification of polymerase chain reaction products. The internal standard method was modified and applied to capillary polymer electrophoresis. The precision of our modified internal standard protocol was evaluated by measuring the relative standard deviation of intermediate capillary polymer electrophoresis experiments. Results showed that the relative standard deviation was reduced from 12.4–15.1 to 0.6–2.3%. Linear regression tests were also implemented to validate our protocol. The modified internal standard method showed good linearity and robust properties. Finally, the ease of our method was illustrated by analyzing a real clinical oral sample using a one‐run capillary polymer electrophoresis experiment. |
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| Keywords: | Capillary polymer electrophoresis Clinical diagnosis Internal standard method Oral bacteria Quantitative analysis |
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