| 对酞内酰胺苯甲酰氯柱前衍生反应HPLC分析痕量多胺 |
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| 引用本文： | 郑明辉,徐宏达.对酞内酰胺苯甲酰氯柱前衍生反应HPLC分析痕量多胺[J].分析测试学报,1993,12(2):42-45. |
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| 作者姓名： | 郑明辉 徐宏达 |
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| 作者单位： | 河北大学理化分析中心 保定
(郑明辉，徐宏达)，河北大学理化分析中心 保定(傅承光) |
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| 摘 要： | 本文提出了一种快速、简便、灵敏检测痕量多胺的新方法。用对酞内酰胺苯甲酰氯同多胺进行柱前衍生反应,以反相高效液相色谱定量测定多胺含量。在4～500 pmol范围内有良好的定量线性关系,腐胺、精脒和精胺的检测限均为0.1 pmol。
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| 关 键 词： | 多胺 高效液相色谱 PIB－CI |
RP-HPLC Determination of Trace Polyamines by Using Pre-column Reaction of 4-(2-phthalimidyl) benzoyl Chloride |
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| Zheng Minghui,Xu Hongda,Fu Chengguang Centre of Physical and Chemical Analysis,Hebei University,Baoding.RP-HPLC Determination of Trace Polyamines by Using Pre-column Reaction of 4-(2-phthalimidyl) benzoyl Chloride[J].Journal of Instrumental Analysis,1993,12(2):42-45. |
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| Authors: | Zheng Minghui Xu Hongda Fu Chengguang Centre of Physical and Chemical Analysis Hebei University Baoding |
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| Institution: | Zheng Minghui,Xu Hongda,Fu Chengguang Centre of Physical and Chemical Analysis,Hebei University,Baoding 071002 |
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| Abstract: | The polyamines e.g. putrescine, spermidine, spermine, and an internal standard, 1,6-diami-nohexane, were derivatized by pre-column reaction of 4-(2-phthylimidyl) benzoyl chloride inpresence of NaHCO_3 and the amides thus produced were separated on an ODS column andthen determined by RP-HPLC with a detection limit of 0.1 pmol. |
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| Keywords: | Polyamines 4-(2-Phthalimidyl) benzoyl chloride HPLC |
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