Agarose-coated anion exchanger prevents cell-adsorbent interactions |
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Authors: | Viloria-Cols Maria E Hatti-Kaul Rajni Mattiasson Bo |
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Institution: | Department of Biotechnology, Center of Chemistry and Chemical Engineering, Lund University, SE-221 00 Lund, Sweden. |
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Abstract: | A common problem during recovery of bioproducts by adsorption from particulate broths is fouling of the adsorbent material as a result of the interaction of cells and cell debris, which present negative charges, with the positively charged anion exchangers commonly used in bioprocesses. The effect of shielding an adsorbent with a layer of agarose on reducing the binding of cells while still allowing the low-molecular-mass bioproducts to be adsorbed was studied. Coating the anion-exchange resin Amberlite IRA-400 with agarose followed by cross-linking the agarose layer effectively prevented the binding of Escherichia coli, Saccharomyces cerevisiae, and Lactobacillus casei cells but allowed binding of lactic acid to the adsorbent. The cross-linked agarose layer was stable during recycling of the adsorbent. |
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Keywords: | Fluidized bed adsorption Fouling Agarose coating Amberlite IRA-400 Cells Adsorbents Lactic acid |
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