Miniaturized planar lipid bilayer: increased stability, low electric noise and fast fluid perfusion |
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Authors: | Tivadar Mach Catalin Chimerel Jürgen Fritz Niels Fertig Mathias Winterhalter Claus Fütterer |
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Institution: | (1) Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany;(2) Nanion Technologies GmbH, Erzgiessereistr. 4, 80335 Munich, Germany;(3) International Center for Transdisciplinary Studies, Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany;(4) INB-1, Forschungszentrum Jülich GmbH, 52425 Jülich, Germany |
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Abstract: | A microfluidic device was designed allowing the formation of a planar lipid bilayer across a micron-sized aperture in a glass
slide sandwiched between two polydimethylsiloxane channel systems. By flushing giant unilamellar vesicles through a 500-μm-wide
channel above the hole, we were able to form a planar lipid bilayer across the hole, resulting in a giga-seal. We demonstrate
incorporation of biological nanopores into the bilayer. This miniaturized system offers noise recordings comparable to open
headstage noise (under 1 pA RMS at 10 kHz), fast precision perfusion on each side of the membrane and the use of nanoliter
analyte volumes. This technique shows a promising potential for automation and parallelization of electrophysiological setups. |
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Keywords: | Microfluidics Planar bilayer Electrophysiology Membrane channel |
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