基于金属介导和离子液体的新型绿原酸印迹整体柱的制备及评价

选用1-乙烯基-3-乙基咪唑四氟硼酸盐(VElm]BF4,一种离子液体)作为功能单体,以Co2+为介导离子,结合1-丁基-3-甲基咪唑四氟硼酸盐(BMIM]BF4)/二甲基亚砜(DMSO)二元致孔体系制备了绿原酸印迹整体柱。经过对制备参数的考察,确定最佳比例为绿原酸:Co2+:VElm]BF4:EDMA(乙二醇二甲基丙烯酸酯)(摩尔比)=1:1:5:20,BMIM]BF4:DMSO=3:1(V/V),最大印迹因子达2.10。通过优化色谱条件,最终在乙腈:20 mmol/L乙酸钠缓冲液(pH 4.2)=70:30(V/V)时实现了绿原酸及其类似物的完全分离。由此可见,以离子液体为功能单体及致孔剂,在金属介导策略下制备的分子印迹聚合物可实现绿原酸的特异性识别及分离。

Preparation and evaluation of a novel chlorogenic acid imprintion monolithic column based on metal-mediation and ionic liquids

In order to develop a new method for preparing chlorogenic acid imprinted monolithic column,1-vinyl-3-ethylimidazolium tetrafluoroborate(VElm]BF4,an ionic liquid)was selected as functional monomer,with Co2+as the mediated ion and 1-butyl-3-methylimidazolium tetrafluoroborate(BMIM]BF4)/dimethyl sulfoxide(DMSO)as binary porogenic system.After examining the preparation parameters,the optimum ratio of chlorogenic acid–cobalt-VElm]BF4–EDMA(ethylene glycol dimethacrylate)was determined as 1:1:5:20(mol/mol/mol/mol),and the ratio ofBMIM]BF4-DMSO was 3:1(V/V).The maximum imprinting factor was 2.10.By optimizing the chromatographic conditions,the complete separation of chlorogenic acid and its analogs was achieved with the mobile phase as acetonitrile:20 mmol/L sodium acetate buffer(pH 4.2)=70:30(V/V).Therefore,the molecularly imprinted polymer prepared with ionic liquid as functional monomer and porogen by metallic pivot strategy could realize the specific recognition and separation of chlorogenic acid.