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| 北虫草多糖的理化特征及抗氧化和免疫活性研究 | |
| 引用本文： | 陈瑞战,殷微,蔡艳,陆娟,田利,王潮雪,谢东雪,杨汝凭,任兴,骆姝君. 北虫草多糖的理化特征及抗氧化和免疫活性研究[J]. 分子科学学报, 2020, 0(2): 160-169,I0006 |
| 作者姓名： | 陈瑞战 殷微 蔡艳 陆娟 田利 王潮雪 谢东雪 杨汝凭 任兴 骆姝君 |
| 作者单位： | 长春师范大学化学学院 |
| 基金项目： | 吉林省教育厅“十三五”科学技术项目(JJKH20190506KJ);长春师范大学自然科学基金资助项目(2019014). |
| 摘 要： | 采取超高压提取,DEAE-Sepharose Fast Flow和Sephadex G-100柱层析分离纯化得到虫草多糖(CMP),通过高效凝胶色谱(HPGPC)、气相色谱(GC)和傅里叶红外光谱(FT-IR)对其理化特征进行了表征,并对CMP抗氧化及免疫调节活性进行了测定.结果显示CMP是纯度较高杂多糖,由鼠李糖、甘露糖、葡萄糖和半乳糖4个单糖组成,结果表明超高压是一种高效的菌多糖提取技术,提取得到的CMP可以探索作为天然的抗氧化和免疫调节剂应用于功能食品和药品. |
| 关 键 词： | 多糖 北虫草 特征 抗氧化活性 免疫调节活性 |
Characterization,antioxidant activity and immunomodulatory activity of polysaccharides from Cordyceps Militaris |
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| CHEN Rui-zhan,YIN Wei,CAI YanLU Juan,TIAN Li,WANG Chao-xue,XIE Dong xue,YANG Ru-ping,REN Xing,LUO Shu-jun. Characterization,antioxidant activity and immunomodulatory activity of polysaccharides from Cordyceps Militaris[J]. Journal of Molecular Science, 2020, 0(2): 160-169,I0006 | |
| Authors: | CHEN Rui-zhan YIN Wei CAI YanLU Juan TIAN Li WANG Chao-xue XIE Dong xue YANG Ru-ping REN Xing LUO Shu-jun |
| Affiliation: | (College of Chemistry,Changchun Normal University,Changchun 130032,China) |
| Abstract: | A homogeneous heteropolysaccharide(CMP)from Cordyceps militaris was extracted through ultrahigh pressure extraction(UPE),separated and purified by DEAE-Sepharose Fast Flow and Sephadex G-100 column chromatography,and its physicochemical properties were investigated by high performance gel permeation chromatography(HPGPC),gas chromatography(GC)and Fourier transform infrared spectrometry(FT-IR),respectively.In addition,the antioxidant and immunostimulatory activities of CMP were investigated.The results showed that CMP was a heteropolysaccharide with excellent purity,mainly composed of rhamnose(Rha),mannose(Man),glucose(Glc)and galactose(Gal).The results indicated that UPE is a high efficient method for extraction bioactive polysaccharides from fungus materials.CMP could be explored as potential natural antioxidant and immunomodulatory agents for applications in medicine or functional food fields. |
| Keywords: | polysaccharid Cordyceps militaris characterization antioxidant activity immunomodulatory activity |
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