An optosensor for tryptophan with C18 silica gel as a substrate |
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| Authors: | Zhilong Gong Z Zhang |
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| Institution: | (1) Department of Chemistry, Nanjing University, Nanjing, China 210008, P.R. China, CN;(2) Department of Chemistry, Shaanxi Normal University, Xian, China 710062, P.R. China, CN |
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| Abstract: | A flow-through optosensor for tryptophan with C18 silica gel as a substrate is proposed. It has been developed in conjunction
with a flow-injection analysis system and is based on the retention of tryptophan on the C18 column and the enhancement of
its fluorescence. The detection limit is 25 ng mL-1 with a relative standard deviation of 2.9% for 7 determinations of 10 μg mL-1 of tryptophan. Most of the common species do not interfere. The method has been successfully applied to a pharmaceutical
preparation.
Received: 8 July 1996/Revised: 26 September 1996/Accepted: 30 September 1996 |
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