Validation of an HPLC method for quantitation of MDMA in tablets |
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Authors: | Müller I B Windberg C N |
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Institution: | Department of Forensic Chemistry, University of Copenhagen, Frederik V's Vej 11, 2100 Copenhagen, Denmark. Irene.breum@forensic.ku.dk |
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Abstract: | An isocratic reversed-phase high-performance liquid chromatography (HPLC) method is developed and validated for the quantitation of 3,4-methylenedioxymethamphetamine (MDMA) in tablets. The chromatographic separation is achieved with potassium phosphate buffer (pH 3.2)-acetonitrile (9:1, v/v) as mobile phase, a Chromspher B column, and UV detection at 210 nm. The calibration curve is linear from 1.4 to 111 microg/mL. The percent relative standard deviation for intra- and interday precision studies is 2.7% each. The measurement uncertainty is estimated to 9%. The method is specific and successfully used for routine quantitation of MDMA in tablets. |
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