Adsorptive stripping voltammetric determination of netilmicin in the presence of formaldehyde |
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Authors: | Nan Sun Weimin Mo Baoxiang Hu Zhenlu Shen |
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Institution: | (1) College of Chemical Engineering and Material Science, Zhejiang University of Technology, Hangzhou, Zhejiang 310032, P.R. China |
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Abstract: | A linear sweep adsorptive stripping voltammetric method for the determination of netilmicin in the presence of formaldehyde
has been proposed for the first time. In the presence of 3.0×10−3 g ml−1 formaldehyde, netilmicin exhibits a sensitive cathodic peak at −1.30 V (vs. the saturated calomel electrode, SCE) in a medium
of Britton–Robinson buffer (pH 8.7) with a scan rate of 100 mV s−1 after a preconcentration period of 120 s at −1.10 V (vs. SCE). The peak current showed a linear dependence on the netilmicin
concentration over the range 4.2×10−9–1.0×10−7 g ml−1. The achieved limits of detection and quantitation were 1.0×10−10 and 3.3×10−10 g ml−1 netilmicin, respectively. It was deduced from the experiments that the amine–aldehyde condensation product formed between
netilmicin and formaldehyde is mainly responsible for the appearance of the peak. The electrochemical behavior of netilmicin
in the presence of formaldehyde has been studied. The method was applied to the direct determination of netilmicin in injectable
formulations and spiked human urine and serum samples.
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Keywords: | Linear sweep adsorptive stripping voltammetry Netilmicin Formaldehyde |
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