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Soil applied cobalt alters the nodulation, leg-haemoglobin content and antioxidant status of Glycine max (L.) Merr
Authors:Jayakumar Kaliyamoorthy  Vijayarengan Packirisamy  Changxing Zhao  Gomathinayagam M  Jaleel Cheruth Abdul
Institution:aEnvironmental Biology Lab, Department of Botany, Annamalai University, Annamalainagar 608002, Tamilnadu, India;bCollege of Plant Science and Technology, Qingdao Agricultural University, Chunyang Road, Chengyang District, Qingdao 266109, China;cStress Physiology Lab, Department of Botany, Annamalai University, Annamalainagar 608002, Tamilnadu, India
Abstract:The present investigation has been carried out to estimate the effect of cobalt on the nodulation leg-haemoglobin content and antioxidant enzyme activities of soybean plants. The experiments were conducted in earthen pots containing 3 kg of air dried soil. The inner surface of pots was lined with polythene sheet. Soybean plants were raised in soil amended with different concentration of cobalt (0, 50, 100, 150, 200 and 250 mg/kg). The root nodule formation, leg-haemoglobin contents and antioxidant enzyme activities were analysed at 15 days intervals, namely 15, 30, 45, 60 and 75 days after sowing (DAS). There found a significant increase in the total number of root nodules, leg-haemoglobin content and antioxidant enzyme activities at 50 mg/kg cobalt application in the soil in all the sampling days when compared to control. Further increase in the cobalt level (100–250 mg/kg) in the soil decreased root nodules formation as well as leg-haemoglobin content antioxidant enzyme activities of soybean plants in all the sampling days.
Keywords:Glycine max  Nodulation  Leg-haemoglobin  Antioxidant enzymes
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