产卵前后罗氏沼虾输卵管的显微和超微结构

利用光镜和电镜技术对罗氏沼虾(Macrobrachium rosenbergii)在产卵前后的输卵管结构进行了观察.输卵管由管壁上皮和基膜组成.管壁上皮为一单层柱状上皮细胞,附着在基膜上.电镜下上皮细胞有明暗之分,细胞面向管腔伸出长而密集的微绒毛.细胞核呈椭圆形,核仁一个或者若干,细胞质内含有丰富的线粒体和粗面内质网.产卵前细胞结构完整,核仁明显,管腔内有大量分泌物;产卵后,游离面细胞膜破碎,微绒毛断裂,细胞内含物排向管腔,管腔内残留少量分泌物.

Observation on microstructure and ultrastructure of the oviduct of Macrobrachium Rosenbergii (Crustacea, Decapoda)

The structure of the oviduct of female Macrobrachium rosenbergii during spawning was examined with optical microscope and transmission electron microscope(TEM).In Macrobrachium rosenbergii,a pair of oviducts originates from the anterolateral part of the ovary ends at the female genital pores situated in the basipodite of the 3rd thoracic appendage.The oviduct wall,folded in some areas, consists of a basal lamina and a layer of columnar epithelial cells.There are two types of epithelial cells,one with light cytoplasm and the other with dark cytoplasm,found in the oviduct wall.The free surface of the epithelial cells possesses long dense microvilli.And beneath the microvilli,there is a thick layer of secreted substance.The nucleus is in oval shape with one or several nucleoli.The cytoplasm contains abundant mitochondria and rough endoplasmic reticulums.After shedding the eggs,the cell membrane and the microvilli break and the cytoplasm leaks out.The layer of secreted substance disappears,only a little residue of it remains.