Prediction of the chromatographic behaviour for a series of diuretic compounds |
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Authors: | J Barbosa R Bergés V Sanz-Nebot |
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Institution: | (1) Departament de Química Analítica, Universitat de Barcelona, Diagonal, 647, 08028 Barcelona, Spain |
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Abstract: | Summary The proportion of organic modifier and the pH of the acetonitrile-water mixtures used as mobile phases were optimized in order
to separate a group of diuretic compounds covering a wide range of physyco-chemical properties. The Linear Solvation Energy
Relationship (LSER) formalism based either on the multiparameter π*, β and α scales or the single solvent polarity parameterE
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, have been used to predict their chromatographic behaviour as a function of the percentage of acetonitrile in the eluent.
Moreover, correlation established between retention and pH of the aqueous-organic mobile phases have been used to predict
the chromatographic behaviour of the diuretic compounds studied as a function of the eluent pH. Linear correlation between
a function of the eluent pH. Linear correlation between the chromatographic retention and theE
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polarity parameter of mobile phases containing different percentages of organic modifier has been obtained Based on the knowledge
of the acid-base dissociation constant the relation between retention and mobile phase pH has also been linearized. These
relationship allowed an important reduction of the experimental retention data needed for developing a given separation and
a great improvement in chromatographic optimization schemes. |
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Keywords: | Column liquid chromatography Retentionvs mobile phase composition Retention prediction Diuretics |
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