A Fluorescent Probe for Diacetyl Detection |
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| Authors: | Xiaohua Li Axel Duerkop Otto S. Wolfbeis |
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| Affiliation: | (1) Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 93040 Regensburg, Germany |
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| Abstract: | A water-soluble fluorescent probe, rhodamine B hydrazide (RBH), was prepared and its properties for recognition of diacetyl were studied. The method employs the reaction of diacetyl with RBH, a colorless and non-fluorescent rhodamine B spiro form derivative to give a pink-colored fluorescent substance. In weakly acidic media, RBH reacts more selectively with diacetyl than with other carbonyls, causing a large increase in fluorescence intensity and thereby providing an easy assay for the determination of diacetyl. |
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| Keywords: | Fluorescent probe Rhodamine B hydrazide Diacetyl Cell medium |
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