| 以四硝基铝酞菁为荧光探针测定葡萄糖的荧光分析新方法 |
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| 引用本文： | 陈贞茂,杨春丽,石立杰,黄萍,李东辉.以四硝基铝酞菁为荧光探针测定葡萄糖的荧光分析新方法[J].分析科学学报,2007,23(5):515-518. |
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| 作者姓名： | 陈贞茂 杨春丽 石立杰 黄萍 李东辉 |
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| 作者单位： | 1. 厦门大学生命科学学院,福建厦门,361005;厦门大学医学院抗癌研究中心,福建厦门,361005
2. 厦门大学医学院抗癌研究中心,福建厦门,361005 |
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| 基金项目： | 国家自然科学基金
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福建省自然科学基金
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厦门大学校科研和教改项目 |
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| 摘 要： | 建立以四硝基铝酞菁为荧光探针测定葡萄糖的荧光分析新方法。方法基于四硝基铝酞菁苯环上的硝基与葡萄糖发生还原反应,形成铝酞菁的氨基化产物;氨基化铝酞菁具有红区激发和发射的特性,在强酸性介质中可发射较强荧光;体系的荧光强度和葡萄糖的含量呈良好的线性关系,并具有较好的准确性和稳定性。方法用于测定发酵样品中的还原糖,结果满意。
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| 关 键 词： | 四硝基铝酞菁 四氨基铝酞菁 葡萄糖 |
| 文章编号： | 1006-6144（2007）05-0515-04 |
| 修稿时间： | 2007-02-06 |
A Novel Method for the Determination of Glucose Using Tetra-Sbistituted Nitro Phthalocyanine as a Fluorescent Probe |
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| CHEN Zhen-mao,YANG Chun-li,SHI Li-jie,HUANG Ping,LI Dong-hui.A Novel Method for the Determination of Glucose Using Tetra-Sbistituted Nitro Phthalocyanine as a Fluorescent Probe[J].Journal of Analytical Science,2007,23(5):515-518. |
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| Authors: | CHEN Zhen-mao YANG Chun-li SHI Li-jie HUANG Ping LI Dong-hui |
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| Institution: | 1. School of Life Sciences, X iamen University, X iamen , Fuj ian 361005; 2. Cancer Research Center ,Medical College, Xiamen University, Xiamen, Fujian 361005 |
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| Abstract: | A novel method has been developed for the fluorimetric determination of glucose using tetra-substituted nitro aluminum phthalocyanine(TNAlPc) as the fluorescence probe.Tetra-substituted nitro aluminum phthalocyanine(TNAlPc) react with glucose,resulting in obvious enhancement in fluorescence in the acidic medium,which is the base of the proposed method.Investigations were carried out on the spectral characteristics,usage of reagent,effect of acidity and the interference of foreign ions.This method has been applied to the determination of practical samples obtained from fermentation liquid. |
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| Keywords: | Tetra-substituted nitro aluminum phthalocyanine Tetra-substituted amino aluminum phthalocyanine Glucose Fluorescence |
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