Imaging of RNA in bacteria with self-ligating quenched probes |
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Authors: | Sando Shinsuke Kool Eric T |
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Institution: | Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA. |
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Abstract: | We report on the application of a new class of oligonucleotide reporter probes, QUAL probes, that "light up" when a nucleophilic phosphorothioate probe binds adjacent to a dabsyl-quenched probe. These self-ligating DNA probes were used for sequence-specific detection of 16S rRNA in Escherichia coli cells. Strong fluorescence was observed only when the phosphorothioate and quenched dabsyl probes bind side-by-side on a 16S rRNA target. The results demonstrate the use of QUAL probes to detect specific RNA sequences in bacterial cells without enzymes and without washing steps. |
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