光镊拉曼光谱法分析红法夫酵母内虾青素含量

建立一种用激光镊子拉曼光谱法快速定量红法夫酵母细胞内虾青素含量的方法。测定不同浓度虾青素标准品溶液的拉曼光谱,取其1 520cm-1峰峰高绘制虾青素标准曲线;取不同氮源、碳源的红法夫酵母细胞,一部分用激光镊子拉曼光谱法测定,一部分用紫外-可见分光光度法测定;最后分析两者间的相关性。结果表明虾青素标准曲线的相关系数到达0.998 3。在单位质量红法夫酵母虾青素含量与单位体积红法夫酵母发酵液虾青素产量方面,对比激光镊子拉曼光谱法与紫外-可见分光光度法,两种方法所得数据具有良好的相关性,其相关系数分别达到0.917 7和0.905 4,表明激光镊子拉曼光谱法能够达到紫外-可见分光光度法的测定效果,是定量分析红法夫酵母细胞内虾青素的更有效方法。

Analysis of Astaxanthin in Phaffia Rhodozyma Using Laser Tweezers Raman Spectroscopy

In the present paper,a method was established based on laser tweezer Raman spectroscopy for rapid quantification of astaxanthin in Phaffia Rhodozyma cells.First,the Raman spectra of astaxanthin standard solution with different concentrations were determined and the standard curve for astaxanthin with the peak intensity at 1 520 cm-1 was plotted;And then the Phaffia yeast cells cultivated in different nitrogen source and carbon source medium were divided into two parts,one for the detection of Raman spectra,and the other for the determination of ultraviolet visible spectrophotometry;Finally the relationship between the two methods was analyzed.The correlation coefficient of standard curve for astaxanthin is 0.998 3.Comparing laser tweezers Raman spectroscopy method with traditional ultraviolet visible spectrophotometry in analyzing the content of astaxanthin in unit mass Phaffia rhodozyma and the yield of astaxanthin in unit volume fermentation broth of Phaffia rhodozyma,the authors found that the data obtained have good linear relationship.And the correlation coefficients are 0.917 7 and 0.905 4,respectively.Therefore,both methods have almost the same effect of measuement.But laser tweezers Raman spectroscopy method is more efficient in the quantitative analysis of astaxanthin in Phaffia Rhodozyma cells.